Application of the DKMG/EGFRvIII cell line opens new perspectives in EGFRvIII research. EGFRvIII expression was reinduced in cells, which expressed EGFRvIII in vivo, but due to unknown reasons did not in vitro. DKMG cells show mutation at DNA level, which is responsible for the expression of EGFRvIII mRNA and protein, however, in most of these cells the expression of mutatant EGFR gene copies is silenced (these cells show the amplification of this gene). The performed biotechnological modification results in the EGFRvIII expression in all cells of the DKMG cell line.